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CD272 (BTLA), PerCP-eFluor™ 710, clone: 6F7, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS46595080

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Catalog No. 501129067



Description

Description

BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells. Like PD1 . and CTLA4 ., BTLA interacts with a B7 homolog, B7H4.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells. Like PD1 . and CTLA4 ., BTLA interacts with a B7 homolog, B7H4.
Specifications

Specifications

CD272 (BTLA)
Monoclonal
0.2 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q7TSA3
BTLA1, CD272, B and T lymphocyte attenuator, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
6F7
PerCP-eFluor™ 710
Btla
Liquid
Btla
IgG1, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
208154
Documents

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