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CD252 (OX40 Ligand) Rat anti-Mouse, PE, Clone: RM134L, eBioscience™

Rat Monoclonal Antibody

Manufacturer:  Life Technologies LS12590582

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Catalog No. 501129187



Description: The RM134L monoclonal antibody reacts with mouse CD252 also known as OX-40 Ligand, a member of the TNF superfamily. OX-40L is induced on mouse splenic B cells stimulated with a combination of anti-IgM and anti-CD40. Neither resting nor activated mouse T cells express OX-40L. The interaction of OX-40 (CD134) with OX-40L has been implicated in T cell-dependent humoral response. RM134L inhibits the costimulatory activity of mouse OX-40L transfected cells for anti-CD3-stimulated T cell proliferation. Applications Reported: This RM134L antibody has been reported for use in flow cytometric analysis. Applications Tested: The RM134L antibody has been tested by flow cytometric analysis of activated mouse splenocytes and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predomitly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNgamma. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.


CD252 (OX40 Ligand)
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Ath-1, Ath1, TXGP1, Txgp1l, gp34, TNFSF4, CD252, OX40 Ligand
Affinity chromatography
Flow Cytometry
IgG2b, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!

For Research Use Only.