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CD244 Monoclonal Antibody (eBioC1.7 (C1.7)), Super Bright™ 436, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Supplier: Thermo Fisher Scientific 62583842
Description
Description: The eBioC1.7 monoclonal antibody reacts with human CD244 (2B4, p38). In human, CD244 is a 38 kDa protein expressed on NK cells, a subset of CD8+ T cells, gammadelta T cells, monocytes, basophils and eosinophils. Binding of the CD244 ligand, CD48, results in NK cell activation, unlike mouse CD244, which is an inhibitory receptor. For CD244 expressed on NK cells, binding of CD48 results in enhanced NK cell cytotoxicity and secretion of IFN-gamma. Recently, it was demonstrated that binding of the C1.7 monoclonal antibody and CD48 involve the same residue in the V domain of human CD244, which explains the ability of C1.7 binding to induce activation of NK cells. Binding of C1.7 to CD244 leads to tyrosine phosphorylation and recruitment of the adaptor molecule SAP (SLAM-associated protein). Patients with X-linked lymphoproliferative disorder (XLPD) have a mutation in SAP which renders it unable to bind to phosphorylated CD244. Applications Reported: This eBioC1.7 (C1.7) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioC1.7 (C1.7) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
CD244 Monoclonal antibody specifically detects CD244 in Human samples. It is validated for Flow Cytometry.Specifications
CD244 | |
Monoclonal | |
5 μL/Test | |
PBS with BSA and 0.09% sodium azide; pH 7.2 | |
Q9BZW8 | |
CD244 | |
Affinity chromatography | |
RUO | |
51744 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
eBioC1.7 (C1.7) | |
Super Bright 436 | |
CD244 | |
2B4; C9.1; CD244; CD244 long isoform; CD244 molecule; Cd244 molecule, natural killer cell receptor 2B4; CD244 natural killer cell receptor 2B4; CD244 short isoform; F730046O15Rik; h2B4; Ly90; NAIL; natural killer cell receptor 2B4; NK cell activation inducing ligand NAIL; NK cell activation-inducing ligand; NK cell receptor 2B4; NK cell type I receptor protein 2B4; NKR Nmrk; NKR2B4; Nmrk; non MHC restricted killing associated; non-MHC restricted killing associated; Signaling lymphocytic activation molecule 4; SLAM family member 4; SLAMF4; transmembrane NK cell receptor 2B4 | |
Mouse | |
100 Tests | |
Primary | |
Human | |
Antibody | |
IgG1 κ |