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CD235a (Glycophorin A) Mouse anti-Human, PE, Clone: HIR2 (GA-R2), eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS12998780

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Catalog No. 501129468



Description

Description

Description: The HIR2 monoclonal antibody reacts with human glycophorin A, sialoglycoproteins expressed by erythroid precursors and mature circulating red cells. Applications Reported: The HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis. Applications Tested: The HIR2 (also GA-R2) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.015 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Glycophorins A (GPA) and B (GPB) which are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.
Specifications

Specifications

CD235a (Glycophorin A)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P02724
Glycophorin A
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
HIR2 (GA-R2)
PE
GYPA
Liquid
GYPA
IgG2b, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
2993
Documents

For Research Use Only.