CD205 Monoclonal Antibody (205yekta), APC, eBioscience™, Invitrogen™

Description

Description: The 205yekta antibody reacts with mouse CD205, also known as DEC-205 (dendritic and epithelial cells, 205 kDa). CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells. CD205 has been shown to bind to PE-Cy5.5 antibody and streptavidin conjugates; therefore, this format should not be used on CD205+ dendritic cells. Applications Reported: This 205yekta antibody has been reported for use in flow cytometric analysis. Applications Tested: This 205yekta antibody has been tested by flow cytometric analysis of in vitro-cultured bone marrow-derived dendritic cells or mouse bone marrow cells. This can be used at less than or equal to 0.03 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD205 Monoclonal antibody specifically detects CD205 in Mouse samples. It is validated for Flow Cytometry.