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CD20, PE-Cyanine5.5, clone: 2H7, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS35020942

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Catalog No. 501129133



Description

Description

CD20 is a cell surface 33-37 (depending on the degree of phosphorylation)kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.

Excitation: 488-561 nm; Emission: 690 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most maligt B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell maligcies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
Specifications

Specifications

CD20
Monoclonal
5 μL/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P11836
B1, Bp35, CD20, LEU-16, MGC3969, MS4A1
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
2H7
PE-Cyanine5.5
MS4A1
Liquid
MS4A1
IgG2b, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
931
Documents

For Research Use Only.