CD1d, Alexa Fluor 488, clone: 1B1, eBioscience™
Rat Monoclonal Antibody
Manufacturer: Life Technologies LS53001182
CD1d is a 49kD heavy chain associated with Beta 2 microglobulin on cortical thymocytes. Beta 2 microglobulin independent expression of CD1d has also been demonstrated on human intestinal epithelial cells. CD1D is the sole group 2 member of the CD1 family of major histocompatibility (MHC) like glycoproteins.Description: The 1B1 monoclonal antibody reacts with mouse CD1d, a 48kDa glycoprotein with structural homology to MHC class I molecules. While similar to MHC Class I, CD1d associates with beta2m, functionally CD1d is similar to MHC Class II. 1B1 detects CD1d at varying levels on mouse leukocytes. 1B1 detects beta2m associated CD1d.Applications Reported: This 1B1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 1B1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|AI747460, CD1.1, Cd1a, Cd1d, Ly-38, T-cell surface glycoprotein CD1d1, antigen-presenting glycoprotein CD1d1|
|Alexa Fluor 488|
|IgG2b, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.