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CD19 Monoclonal Antibody (eBio1D3 (1D3)), Super Bright™ 645, eBioscience™, Invitrogen™ DFS Item

Rat Monoclonal Antibody

Supplier:  Thermo Fisher Scientific 64019382

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Catalog No. 64019382



Description

Description

Description: The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express this molecule. CD19, along with CD21, CD81, Leu13, and MHC class II, form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes. Applications Reported: This SJ25C1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This SJ25C1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No.

CD19 Monoclonal antibody specifically detects CD19 in Mouse samples. It is validated for Flow Cytometry.
Specifications

Specifications

CD19
Monoclonal
0.2 mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P25918
Cd19
Affinity chromatography
RUO
12478
4° C, store in dark, DO NOT FREEZE!
Liquid
Flow Cytometry
eBio1D3 (1D3)
Super Bright 645
Cd19
AW495831; B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Cd19; CD19 antigen; CD19 molecule; CVID3; differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12
Rat
100 μg
Primary
Mouse
Antibody
IgG2a κ
SDS
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