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CD16 Mouse anti-Human, Non-human primate, PerCP-eFluor 710, Clone: 3G8, eBioscience™

Mouse Monoclonal Antibody

$216.05 - $477.05

Specifications

Antigen CD16
Clone 3G8
Host Species Mouse
Gene Alias FcgRIII, FcgR3, ADCC receptor
Species Reactivity Baboon, Chimpanzee, Cynomolgus, Human, Macaque, Monkey, Pigtailed Macaque, Rhesus Ma
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 Disclaimers

For Research Use Only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

5015927

 
affymetrix
46-0166-41
25 tests Each for $216.05

5015928

 
affymetrix
46-0166-42
100 tests Each for $477.05
Description & Specifications

Specifications

Antigen CD16
Clone 3G8
Host Species Mouse
Gene Alias FcgRIII, FcgR3, ADCC receptor
Species Reactivity Baboon, Chimpanzee, Cynomolgus, Human, Macaque, Monkey, Pigtailed Macaque, Rhesus Ma
Applications Flow Cytometry
Regulatory Status RUO
Conjugate PerCP-eFluor 710
Format Conjugated
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 5μL (0.125μg)/test

This 3G8 monoclonal antibody reacts with human and non-human primate CD16, which is also known as the low-affinity Fc gamma RIII. CD16 exists as two distinct isoforms, Fc gamma RIIIA and Fc gamma RIIIB. In humans, Fc gamma RIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as NK cells. T and B cells do not express this Fc receptor. Fc gamma RIIIB is also detected on neutrophils as a GPI-anchored form. Expression of CD16 on lymphocytes and monocytes is similar in non-human primates. However, while CD16 is not found on neutrophils in macaques and baboons, this receptor is detected on these cells in sooty mangabeys. Binding of IgG leads to activation of signal transduction pathways, resulting in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation.

This monoclonal antibody has been reported to have several functional activities, including inhibition of cytotoxic ability, activation of cell signaling, and NK cell depletion in vivo. Moreover, the 3G8 antibody clone has been demonstrated to work on capuchin monkey, chimpanzee, common marmoset, cynomologous monkey, hamadyras baboon, olive baboon, pigtailed macaque, rhesus, and squirrel monkey.

Based on cross-blocking studies 3G8 recognizes the same epitope as CB16. However, 3G8 and B73.1 antibody clones bind distinct epitopes.