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CD16 Mouse anti-Human, Functional Grade, Clone: B73.1, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS16016785

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Catalog No. 501128688



Description

Description

Description: This B73.1 monoclonal antibody reacts with human CD16, a member of the Fc receptor family also known as Fc gamma RIIIa and Fc gamma RIIIb. This 50-65 kDa receptor is expressed on natural killer cells, neutrophils, monocytes, and macrophages. Crosslinking of CD16 in natural killer cells and monocytes results in activation of the MAPK and PI3K signaling pathways, thus regulating the production of TNF alpha, IL-6, and IL-1 beta. This monoclonal antibody has been shown to block Fc receptor functions. Applications Reported: This B73.1 antibody has been reported for use in functional blocking assays and flow cytometric analysis. Applications Tested: This B73.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC. CD16 (FCGR3A) is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Human CD16 is expressed in two forms – FCGR3A and FCGR3B. FCGR3A is associated with the FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FCGR3A is associated, with FcepsilonRI-beta subunit. Besides IgG, FCGR3A can be triggered also by oligomeric IgE. FCGR3B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a pro-adhesive phenotype. Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.
Specifications

Specifications

CD16
Monoclonal
1 mg/mL
PBS with no preservative; pH 7.2
O75015, P08637
FCGR3A, FCGR3B, CD16A, CD16B, Fc gamma Receptor III, Fc gamma Receptor 3, RP11-5K23.1, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry, Functional Assay, Neutralization
B73.1
Functional Grade
FCGR3A
Liquid
FCGR3A, FCGR3B
IgG1, kappa
500 μg
4° C
Primary
2214, 2215
Documents

For Research Use Only.