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CD15, FITC, clone: HI98, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS11015942

 View more versions of this product

Catalog No. 501129432



Description

Description

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.

Description: The HI98 monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative.Applications Reported: The HI98 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI98 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD15
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
stage-specific embryonic antigen 1, CD15, SSEA-1, SSEA1, ELFT, FCT3A, FUC-TIV, FUTIV, LeX
IgM, kappa, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
Human
Flow Cytometry
HI98
FITC
Liquid
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Documents

For Research Use Only.