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CD140a (PDGFRA) Rat anti-Mouse, Biotin, Clone: APA5, eBioscience™

Rat Monoclonal Antibody

Manufacturer:  Life Technologies LS13140182

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Catalog No. 501128704



Description

Description

Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. Applications Reported: The APA5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
Specifications

Specifications

CD140a (PDGFRA)
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P26618
PDGF Receptor alpha, MGC74795, PDGFR2, RHEPDGFRA, CD140 antigen-like family member A, CD140a antigen, PDGF-R-alpha, PDGFRA/BCR fusion, pdgfr-a
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
APA5
Biotin
Pdgfra
Liquid
Pdgfra
IgG2a, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
18595
Documents

For Research Use Only.