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CD137 (4-1BB) Mouse anti-Human, PE-Cyanine7, Clone: 4B4 (4B4-1), eBioscience™ DFS Item

Mouse Monoclonal Antibody

$214.00 - $491.00

Specifications

Antigen CD137 (4-1BB)
Clone 4B4 (4B4-1)
Target Species Human
Conjugate PE-Cyanine7
Isotype IgG1, kappa
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
25137941 View Documents Life Technologies
LS25137941
25 Tests Each for $214.00
 
25137942 View Documents Life Technologies
LS25137942
100 Tests Each for $491.00
 
Description

Description

Description: This 4B4 (4B4-1) monoclonal antibody reacts with human CD137 (also known as 4-1BB or TNFRSF9), which is an inducible member of the TNFR family of costimulatory molecules expressed on T cells, natural killer cells, dendritic cells, granulocytes, and mast cells. Involved in recruiting TNFR-associated factors (TRAF) 1 and 2, CD137 signaling plays a role in T cell activation, maintaining the survival of activated and CD8 memory T cells, as well as suppressing myelopoiesis and dendritic cell development. Stimulation of this receptor has also been shown to promote expansion of CD4+CD25+ T regulatory cells i{ex vivoi}. The ligand for CD137, 4-1BBL, is found on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. Applications Reported: This 4B4 (4B4-1) antibody has been reported for use in flow cytometric analysis. Applications Tested: This 4B4 (4B4-1) antibody has been tested by flow cytometric analysis on two-day Con A-stimulated normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation.

Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB.
Specifications

Specifications

CD137 (4-1BB)
Human
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
4B4 (4B4-1)
PE-Cyanine7
Flow Cytometry
Primary
Monoclonal
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
RUO