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CD133 (Prominin-1), PerCP-eFluor™ 710, clone: TMP4, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS46133842

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Catalog No. 501129278



Description

Description

The CD133 (also named AC133) antigen is a 97kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120kDa band on protein gels due to glycosylation. It is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. It has been shown to be expressed in cancers, including some leukemias and brain tumours, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver.

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. The CD133 (also named AC133) antigen is a 97 kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120 kDa band on protein gels due to glycosylation. It is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. It has been shown to be expressed in cancers, including some leukemias and brain tumours, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver.
Specifications

Specifications

CD133 (Prominin-1)
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
O43490
AC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
TMP4
PerCP-eFluor™ 710
PROM1
Liquid
PROM1
IgG1, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
8842
Documents

For Research Use Only.