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CD117 (c-Kit), Super Bright 645, clone: 2B8, eBioscience™ DFS Item

Rat Monoclonal Antibody

$231.00 - $471.25

Specifications

Antigen CD117 (c-Kit)
Clone 2B8
Target Species Mouse, Porcine
Conjugate Super Bright 645
Isotype IgG2b, kappa
View More Specs

Products 2 Description

Description

c-Kit, also known as CD117 and stem cell factor receptor, is a 145kDa transmembrane tyrosine kinase encoded by the c-Kit proto-oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. c-Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Phosphorylation at tyrosine 703 of c-Kit allows binding of Grb2 and activation of the Ras-Raf-ERK1 and 2 signaling pathway.

Description: The 2B8 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor, and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145-kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1 low) and negative for lineage markers. The interaction of the mouse c-Kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells.Applications Reported: This 2B8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 2B8 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^9 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product # SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser
Specifications

Specifications

CD117 (c-Kit)
Mouse, Porcine
IgG2b, kappa
Monoclonal
Liquid
Antibody
4°C, store in dark, DO NOT FREEZE!
2B8
Super Bright 645
Flow Cytometry
Primary
Monoclonal
PBS with BSA and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only