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CD117 (c-Kit) Monoclonal Antibody (104D2), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Supplier: Invitrogen 46117842
Description
Description: The 104D2 monoclonal antibody reacts with human CD117, also known as c-Kit , Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells. Applications Reported: This 104D2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 104D2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat.
CD117 (c-Kit) Monoclonal antibody specifically detects CD117 (c-Kit) in Human samples. It is validated for Flow Cytometry.Specifications
CD117 (c-Kit) | |
Monoclonal | |
5 μL/Test | |
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
P10721 | |
KIT | |
Affinity chromatography | |
RUO | |
3815 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
104D2 | |
PerCP-eFluor 710 | |
KIT | |
belly-spot; Bs; CD117; ckit; C-Kit; c-KIT gene1; c-kit protein; c-kit proto-oncogene protein; c-kit receptor; c-kit receptor tyrosine kinase; dominant spotting; Dominant white spotting; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; KIT; kit oncogene; KIT proto-oncogene receptor tyrosine kinase; KIT proto-oncogene, receptor tyrosine kinase; mast cell growth factor receptor; mast/stem cell growth factor receptor; mast/stem cell growth factor receptor Kit; MGF; p145 c-kit; PBT; Piebald trait protein; protein kinase; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; receptor tyrosine kinase c-kit; receptor tyrosine kinase type III; SCFR; SCO1; SCO5; Sl; soluble KIT variant 1; SOW3; spotted sterile male; Ssm; Steel Factor Receptor; Tr-kit; tyrosine kinase receptor; tyrosine kinase receptor c-KIT; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; W | |
Mouse | |
100 Tests | |
Primary | |
Human | |
Antibody | |
IgG1 κ |
For Research Use Only.