CD107a (LAMP-1) Monoclonal Antibody (eBio1D4B (1D4B)), eFluor™ 660, eBioscience™, Invitrogen™

Description

Description: The eBio1D4B monoclonal antibody reacts with mouse CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a type I, lysosomal membrane protein that is extensively glycosylated. It is expressed constitutively in the late endosomes-lysosomes in all cells. CD107a is also transiently expressed on the cell surface of degranulating cytolytic T cells. Additionally, CD107a has been implicated in a variety of cellular functions including cancer metastasis and is also a marker for lysosomal storage disorders. Applications Reported: This eBio1D4B (1D4B) antibody has been reported for use in intracellular staining and flow cytometric analysis. It has also been reported for use in surface staining in a flow cytometric based degranulation assay. Applications Tested: This eBio1D4B (1D4B) antibody has been tested by intracellular staining and flow cytometric analysis of mouse thioglycolate-induced peritoneal exudate cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm).

CD107a (LAMP-1) Monoclonal antibody specifically detects CD107a (LAMP-1) in Human, Mouse samples. It is validated for Flow Cytometry.