CD10 Monoclonal Antibody (SN5c), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

Description: The eBioSN5c monoclonal antibody recognizes human CD10 (CALLA, NEP, enkephalinase, Neprilysin), which is a 100 kDa, type II cell surface glycoprotein originally identified for its expression on most acute lymphoblastic leukemias (ALLs). Subsequently, CD10 was shown to be the same molecule as neutral endopeptidase (NEP), or KII-NA. CD10 is a Zn^2+-dependent metallopeptidase acting on substrates such as endothelin, glucagon, gastrin, neurotensin and bradykinin. CD10 is involved in the regulation of chemotactic and inflammatory processes involving neutrophils. In B cells, CD10 regulates stromal cell-dependent B lymphopoiesis and its expression has been reported on mature germinal center B cells. CD10 expression is also found on normal donor granulocytes and bone marrow stromal cells. Most likely, CB-CALLA and eBioSN5c see different epitopes due to their ability to co-stain. Applications Reported: This eBioSN5c (SN5c L4-1A1) antibody has been reported for use in flow cytometric analysis. Applications Tested: This SN5c antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.