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Caspase 8 Rat anti-Human, Clone: 1H10, eBioscience™

Rat Monoclonal Antibody

$143.55 - $332.05

Specifications

Antigen Caspase 8
Clone 1H10
Host Species Rat
Gene Alias Caspase8
Species Reactivity Human
View More Specs

 Disclaimers

For Research Use Only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

5013892

 
affymetrix
14993480
25μg Each for $143.55

5013893

 
affymetrix
14993482
100μg Each for $332.05
Description & Specifications

Specifications

Antigen Caspase 8
Clone 1H10
Host Species Rat
Gene Alias Caspase8
Species Reactivity Human
Applications Western Blotting
Regulatory Status RUO
Conjugate Unlabeled
Format Purified
Storage Requirements Store at 2-8°C.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 0.5mg/mL

The monoclonal antibody 1H10 recognizes human Caspase-8 also known as FLICE and MACH, a member of the cysteine-requiring aspartate protease family. Caspase-8 is an upstream caspase responsible for Fas and TNF-α mediated apoptosis. After activation of the Fas or TNF receptors by their ligands, the adaptor molecule FADD binds to the receptor and recruits caspase-8 to the membrane. The formation of this complex induces the cleavage and activation of caspase-8. The active form of caspase-8, made of a heterotetramer of p18 and p10, is released in the cytoplasm and activates downstream apoptotic proteases (caspase-3, -4, -6, -7, -9 and -10). Several (> 8) isoforms have been identified: 3 different pro-forms called Caspase-8a (55kDA), Caspase-8b (53kDa), and Caspase-8L which is procaspase-8 with an additional N-terminal extension of 59 aa.
The 1H10 antibody recognizes the p55 and p53 proforms in addition to the p43 and p18 cleaved forms. Under reducing conditions, an additional band (possibly non-specific) around 35kDa may be present. Under nonreducing conditions, this band is absent.