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CAMK2A (CaMKII Alpha) Recombinant Human Protein DFS Item

CAMK2A (CaMKII alpha)

Manufacturer:  Life Technologies LSPV3142

Catalog No. PV3142



CAMK2A (CaMKII alpha) is a serine⁄threonine kinase that regulates a number of cellular functions including neuronal processes, gene expression, carbohydrate metabolism, cytoskeletal function, and calcium homeostatsis. Autophosphorylation of threonine 286 in the presence of Ca2+ and calmodulin activates CAMK2A with the ability to switch from calmodulin-dependent to a calmodulin independent activity.


Pharma and biopharma, protein assays and analysis, target and lead identification and validation
90% by SDS-PAGE
1 Each
CAMK2A (CaMKII alpha)
CAMK2A (CaMKII Alpha) was pre-diluted in enzyme dilution buffer and assayed in 10mM HEPES (pH 7.5), 10mM MgCl2, 1mM Na3VO4, 10μg/mL calmodulin, 0.5mM CaCl2, 5mM DTT, 100 μM ATP, 50 μM Autocamtide-2 peptide substrate (KKALRRQETVDAL) and trace [32P]-γ- ATP for 10 minutes at 30°C
Kinases and inhibitors
CAMK2 Kinase family
7, 460nmoles of phosphate transferred to Autocamtide-2 peptide substrate (KKALRRQETVDAL) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.05μg/mL
His Tag
25400, 815, 816, 817, 818
CAMK2A (CaMKII Alpha) Recombinant Human Protein
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
CAMK2A (CaMKII Alpha) Recombinant Human Protein
Kinase assay
CAMK2A (CaMKII Alpha) Recombinant Human Protein

For Research Use Only. Not for use in diagnostic procedures.