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eBioscience™ Calcein AM Viability Dye, UltraPure Grade

Membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein.

$183.48 - $282.48


For Use With (Application) Flow Cytometric Analysis, Microscopy
Format Lyophilized off-white solid
Storage Requirements −20°C. Protect from light and moisture.
View More Specs


For Research Use Only

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Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
5016952 Life Technologies
1mg Each for $183.48
5016953 Life Technologies
20 x 50μg Each for $282.48


  • Molecular weight: 994.86g/mol
  • Peak excitation: 495nm
  • Peak emission: 515nm
  • Reconstitute in high-quality, freshly opened DMSO
  • Once reconstituted, store at −20°C with dessicant and use within a short period of time. Avoid freeze-thawing
  • For fluorescent microscopy, detect using the appropriate filter sets
  • For flow cytometric analysis, excite with the blue laser line (488nm) and detect using filters for FITC/Alexa Fluor™ 488 (530/30)
  • Co-staining with Annexin V or 7-AAD is recommended to allow the greatest resolution between live and dead/apoptotic cells
  • If single-color analysis is desired, improved resolution between live and dead/apoptotic cells may be obtained by using either Calcein Blue AM (cat. 65-0855) or Calcein Violet 450 AM (cat. 65-0854)
Description & Specifications


Flow Cytometric Analysis, Microscopy
Lyophilized off-white solid
−20°C. Protect from light and moisture.