MilliporeSigma™ Calbiochem™ InSolution™ Y-27632 in DMSO

Description

A highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (K_i = 140nM for p160^ROCK). Also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP. Exhibits 10- to 50-fold lower affinity for PKC_ε than p160^ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at higher concentrations (∽100μM). Also acts as a potent inhibitor of agonist-induced Ca²⁺ sensitization of myosin phosphorylation and smooth muscle contraction. Prevents apoptosis and enhances the survival and cloning efficiency of dissociated hES cells without affecting their pluripotency. A 5mm (500μg/296μl) solution of Y-27632 (Cat. No. 688001) in H₂O and the solid form of this compound (Cat. No. 688000) are also available.