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Thermo Scientific™ Brij™-35 Detergent Solutions

Stabilized Brij-35 detergent provided as 10% and 30% solutions for use in various protein methods including cell lysis and HPLC.

Manufacturer:  Thermo Scientific™ 85118

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Catalog No. PI85118



Thermo Scientific™ Brij-35 Detergent Solutions are stabilized 10% and 30% solutions of Brij-35 detergent for use in various protein methods.
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Brij-35 is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or as a surfactant in various HPLC applications. The detergent is offered in two forms: a Thermo Scientific Surfact-Amps™ Detergent Solution (high-purity, 10% solution) and a standard grade 30% solution.

Surfact-Amps Detergent Solutions (10% w/v) provide unsurpassed purity, quality and stability. Unlike neet detergents, which are extremely viscous, Surfact-Amps 10% Solutions are easy to pipette and accurately dispense. The surfactant solutions are carefully prepared and packaged under nitrogen in glass ampules, ensuring their stability and eliminating the accumulation of peroxides and degradation products.


  • Brij-35 – a nonionic detergent for use in various protein methods

  • Accurate – 10% and 30% detergent solutions in ultrapure water

  • Easy-to-use – solutions are simple to dispense and dilute for use

Detergent Properties:

  • Molecular Weight: 1225g
  • Detergent Class: Nonionic
  • Aggregation Number: 40
  • Micelle Molecular Weight: 49,000g
  • Critical Micelle Concentration (CMC): 0.09mM (0.011%, w/v)
  • Cloud Point: >100°C
  • Dialyzable: No

Surfact-Amps Detergent Solution (Part No. 28316):

  • Accurate – precise 10% detergent solution in ultrapure water

  • Easy-to-use – solution is simple to dispense and dilute for use

  • Exceptionally pure – less than 1.0μeq/ml peroxides and carbonyls

  • Stable – packed in glass ampules under inert nitrogen gas



10% (w/v) aqueous solution of Brij-35
Brij-35 Surfact-Amps Detergent Solution