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eBioscience BrdU Kit for IHC/ICC Colorimetric

Contains the necessary reagents and buffers for identifying and examining proliferating cells by immunohistochemical or immunocytochemical analysis. Optimized for IHC with both frozen and paraffin-embedded BrdU-labeled mouse intestine and ICC of BrdU-pulsed HeLa cells grown on culture slides.

$433.55 - $738.05

Specifications

Applications Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections, Microscopy, Immunocytochemistry, Immunohistochemical Staining of Frozen Tissue Sections
Storage Requirements Refer to individual components
Species Chimpanzee, Human, Macaque, Mouse, Rat
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Includes:  BrdU Fixation Buffer
BrdU Antigen Retrieval Solution (10X)
BrdU Blocking Solution
Anti-BrdU Biotin (clone BU20A) : 10 μg
Avidin-HRP
3, 3'-Diaminobenzidine (DAB) Substrate Solution: Reagent A (DAB Chromogen), Reagent B (DAB Diluent), and Dropper Bottle (for storage and application of working DAB solution)

 Alert

Contains Sodium azide, formaldehyde, and hydrogen peroxide

Products
Catalog Number Mfr. No. Quantity Price Quantity    

501125060

 
ebioscience
8800659945
50 tests Each for $433.55

501125059

 
ebioscience
8800659942
100 tests Each for $738.05
Description & Specifications

Specifications

Applications Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections, Microscopy, Immunocytochemistry, Immunohistochemical Staining of Frozen Tissue Sections
Storage Requirements Refer to individual components
Species Chimpanzee, Human, Macaque, Mouse, Rat

  • Cells are labeled in vitro or in vivo with 5-bromo-2′-deoxyuridine (BrdU), which is incorporated into DNA in place of thymidine during the S-phase of the cell cycle
  • Following fixation and antigen-retrieval steps, cells or tissue sections are stained for BrdU incorporation and visualized using an Avidin-HRP and DAB substrate reaction