Learn More
Invitrogen™ Bolt™ 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well
Separation Range: 3.5 to 260 kd; Separation Type: Denaturing; For Use With: Mini Gel Tank, XCell SureLock Mini
Manufacturer: Invitrogen™ LSNW04120BOX
Description
Similar to NuPAGE™ Bis-Tris gels, Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt gels are ideal for Western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt Bis-Tris Plus gels to obtain optimal results for day-to-day protein separation needs.
Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and 4–12% gradient. The gels can be run using Bolt MES or MOPS SDS running buffer to obtain different separation ranges. Bolt Bis-Tris Plus gels consist of a neutral pH-buffered (pH 6.4) polyacrylamide gel and have an operating pH of about 7.0. Bolt Bis-Tris Plus gels also come in four different well formats: 10, 12, 15, and 17 wells.
- Optimized run conditions—separate proteins using constant voltage in approximately 35 minutes
- Better protein integrity—neutral pH sample preparation process preserves proteins
- Improved protein separation—offer sharper bands, high resolution, longer shelf life, and higher accuracy of results than tris-glycine—based gels
- Wedge-shaped wells—easier sample loading and up to two times higher sample volume than other 1.0mm-thick gels
Specifications
No Adapters | |
Molecular Weight | |
Denaturing | |
Room Temperature | |
1D well (wedge shaped) | |
4-12% acrylamide | |
8 cm | |
Up to 60 μL/well (40 μL recommended) | |
8 cm | |
Bis-Tris Plus Gel | |
3.5 to 260 kd | |
Bis-Tris | |
1.0 mm | |
Protein | |
1 Box |
Up to 60 µL per well | |
Bolt™ | |
up to 60 µL | |
1.0 mm | |
Day-to-Day Protein Separations, Techniques where Protein Integrity is Crucial, Western Blot Transfer and Analysis | |
8 cm | |
4°C to 25°C | |
8 cm | |
Gradient Page | |
Mini | |
4 to 12% | |
Denaturing | |
Mini Gel Tank,XCell SureLock Mini | |
10-well | |
One box contains 10 gels. Store at 4–25°C. Shelf life is 16 months. |
For Research Use Only. Not for use in diagnostic procedures.