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Thermo Scientific™ Blocker™ Casein Blocking Buffers


Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods.

Manufacturer: thermo scientific™  37582

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Catalog No. PI37582

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Description & Specifications

Specifications

Applications ELISA, IHC, WB
Description Blocker Casein in PBS
Formulation 1% (w/v) casein (Hammarsten grade) in 100mM sodium phosphate, 150mM NaCL, pH 7.4 containing Kathon anti-microbial agent
Quantity 100mL
Sufficient For 10 mini blots at 10mL each

Thermo Scientific™ Blocker Casein Blocking Buffers are ready-to-use, PBS or TBS solutions of purified casein protein for blocking steps in Western blotting, ELISA, immunohistochemistry and nucleic acid detection methods.

These blocking buffers contains casein protein that is purified from milk by the Hammarsten method. The phosphate- and Tris-buffered saline formulations are used for blocking excess binding sites on membranes and microplates in antibody-based detection methods. Blocker Casein Buffers are 1% (w/v) casein, which corresponds to the optimal concentration for most applications.

Highlights:

  • Purified casein – single-protein blocking buffer provides fewer chances of cross-reaction with assay components than serum or milk solutions

  • Easy to use – 1% casein solutions are ready to use; can be diluted further as needed

  • Flexible – available in PBS and TBS formulations to suit a variety of applications

  • Safe – stable, thimerosal-free formulations