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Thermo Scientific™ Blocker™ BSA Blocking Buffers


Block membranes, plates and other samples with these ready-to-use 10X PBS or TBS solutions of bovine serum albumin for Western blot, ELISA and other detection methods.

Manufacturer: thermo scientific™  37525

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Catalog No. PI37525

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Description & Specifications

Specifications

Applications ELISA, IHC, WB
Description Blocker BSA in PBS (10X)
Formulation 10% (w/v) bovine serum albumin in phosphate-buffered saline, pH 7.4, with Kathon preservative
Quantity 200mL
Sufficient For 65 blots or microplates at 1% and 30mL each

Thermo Scientific™ Blocker BSA Blocking Buffers are ready-to-use, 10X PBS or TBS solutions of bovine serum albumin protein for blocking steps in Western blotting, ELISA, immunohistochemistry and nucleic acid detection methods.

These blocking buffers are 10% (w/v) solutions of high-quality BSA that are useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. Blocker BSA Buffers are most frequently diluted 10-fold (to 1% BSA) for initial testing, but other buffer concentrations can be beneficial for specific systems. Blocker BSA is usually more effective than nonfat milk blocking buffers for biotin-avidin systems because it contains a single purified protein that is devoid endogenous biotin.

Highlights:

  • Purified protein – 10% solutions of high-quality bovine serum albumin; a single purified protein provides fewer chances of cross-reaction with assay components than serum or milk solutions

  • Convenient – concentrated formulation saves storage space and can be diluted easily to obtain optimal blocking results for specific applications

  • Easy to use – no waiting for powder to dissolve with this ready-to-dilute liquid concentrate

  • Flexible – available in PBS and TBS formulations to suit a variety of applications