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Biotin Mouse anti-Chemical, Tag, Alexa Fluor® 488, Clone: BK-1/39, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS53989582

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Catalog No. 501129103



Description: This BK-1/39 monoclonal antibody reacts with biotin, a B-complex vitamin. As a coenzyme, biotin is involved in the metabolism of fatty acids, amino acids, and gluconeogenesis. In the laboratory, biotin is used to tag molecules of interest for biochemical or cell biological studies. This monoclonal antibody can be used to detect biotinylated proteins and antibodies, particularly if signal amplification is desired. Applications Reported: This BK-1/39 antibody has been reported for use in flow cytometric analysis. Applications Tested: This BK-1/39 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells stained with a biotin-conjugated antibody. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B4. It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required for incorporation of carbon dioxide into organic compounds and exhibits a MW of ∽244 Da.


0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Vitamin B4
IgG1, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Chemical, Tag
Flow Cytometry
Alexa Fluor® 488
Affinity chromatography

For Research Use Only.