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BioTek™ Synergy™ H4 Hybrid Microplate Reader

Combines two powerful detection systems, monochromator-based and filter-based, in one compact unit

Manufacturer: biotek™  H4MLFAD

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Catalog No. BTH4MLFAD

 Please call Customer Service at 1-800-234-7437 or send an email to for assistance.

Description & Specifications


Light Source Tungsten halogen and xenon flash
Detector Type Absorbance
Excitation Wavelength Range 200 to 700nm
Excitation Filters 4-position wheel with four excitation filters (360/40, 485/20, 530/25, 590/20) and four emission filters (460/40, 528/20, 590/35, 645/40)
Emission Wavelength Range 300 to 700nm (800nm with red extended option)
Linearity >lt;1% from 0 to 3.0 O.D. (typical)
Accuracy ±1% at 2 O.D., typical
Sensitivity 2.5pg/mL SF; typical
Dynamic Range 0 to 99,999 (fluorescence, luminescence); 0 to 4.0 O.D. (absorbance)
Interface Bidirectional RS-232
Width (Metric) 53.1cm
Width (English) 20.9 in.
Height (Metric) 38.1cm
Height (English) 15 in.
Hertz 50/60Hz
Voltage 100-240V
Electrical Requirements 100-240V 50/60Hz
Includes With injectors

Enjoy complete flexibilty and assay choice for current as well as future demands. Advanced multi-detection system is capable of performing an unlimited number of microplate-based assays.

  • Hybrid Technology™: Synergy H4 combines the sensitivity of a filter-based system with the convenience of monochromator-based optics to provide the broadest range of applications available on the market today.
  • Detection modes: Fluorescence Intensity, Time-Resolved Fluorescence, Fluorescence Polarization, AlphaScreen™/AlphaLISA, Luminescence, UV-Visible absorbance, FRET, TR-FRET, BRET, well area scanning and spectral scanning.
  • Modular and upgradeable architecture: Read modes are available as individual modules for cost-effectiveness and peace of mind.
  • Quadruple mono system and variable bandpass selection: Synergy H4 optics incorporates two double-grating monochromators. This quadruple design provides the best in spectral scanning performance and flexibility.
  • Deep blocking filters and dichroic mirrors: Synergy H4's filter/dichroic combination provides the best possible performance in fluorescence, time resolved fluorescence and fluorescence polarization applications.
  • Compatible with Take3 Plate with 2μL microspots: Enables low volume 260nm nucleic acid quantification

Synergy H4 Monochromator System

  • Uses two double-grating monochromators with variable bandpass selection
  • Highest stray light reflection, continuous wavelength selection, spectral scanning
  • Combines high performance with convenience and flexibility
Synergy H4 Filter/Mirror System
  • Delivers more energy to the sample and provides high signal-to-noise rations
  • Faster read speed, more sensitivity, more precise control over optical parameters