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BD Difco™ Yeast Nitrogen Base without Amino Acids and Ammonium Sulfate

Used in molecular genetics for classifying yeasts based on carbon and nitrogen requirements. BD™ Difco™ Yeast Nitrogen Base without Amino Acids and Ammonium Sulfate is prepared according to the formulas of Wickerham.

Manufacturer:  BD B233510

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Catalog No. DF0335-08-8




BD Difco Yeast Nitrogen Base without Amino Acids and Ammonium Sulfate; 10kg pail; Dehydrated Media, Culture; Wickerham formula, classification of yeasts based on nitrogen and carbon requirements

Media with Yeast Nitrogen Base without Amino Acids and Ammonium Sulfate are included in many applications for the study of yeasts in molecular genetics. Yeasts are unicellular, eukaryotic, budding cells that are generally round-to-oval or elongate in shape. They multiply principally by the production of blastoconidia (buds). Yeast colonies are moist and creamy or glabrous to membranous in texture. Yeasts are considered opportunistic pathogens.

  • Contains all essential nutrients and vitamins necessary for the cultivation of yeasts and a source of nitrogen and carbohydrate
  • Lacks the amino acids histidine, methionine and tryptophan and ammonium sulfate
  • Prepared according to Guenter’s modification of Wickerham’s Yeast Nitrogen Base formulation
  • Displays a good cultural response to Kloeckera apiculata 9774 and Saccharomyces cerevisiae 9080
  • pH (0.17% solution, 25°C): 4.5 ± 0.2
Description & Specifications


ISO/DIS, 11014
For classifying Yeasts based on colonial characteristics and cell morphology
Approximate Formula per Liter:
  • Vitamins: Biotin 2μg/L, Calcium Pantothenate 400μg/L, Folic Acid 2μg/L, Inositol 2,000μg/L, Niacin 400μg/L, p-Aminobenzoic Acid 200μg/L, Pyridoxine Hydrochloride 400μg/L, Riboflavin 200μg/L, Thiamine Hydrochloride 400μg/L
  • Compounds Supplying Trace Elements: Boric Acid 500μg/L, Copper Sulfate 40μg/L, Potassium Iodide 100μg/L, Ferric Chloride 200μg/L, Manganese Sulfate 400μg/L, Sodium Molybdate 200μg/L, Zinc Sulfate 400μg/L
  • Salts: Monopotassium Phosphate 1.0g, Magnesium Sulfate 0.5g, Sodium Chloride 0.1g, Calcium Chloride 0.1g
Light yellowish-beige
Dehydrated, powder
Yeast Nitrogen Base
Yeast Nitrogen base without Amino Acids and Ammonium Sulfate