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BD™ Difco™ Yeast Nitrogen Base without Amino Acids

Used for classifying yeasts based on amino acid and carbohydrate requirements. BD™ Difco™ Yeast Nitrogen Base without Amino Acids is a dehydrated culture medium used in many molecular genetics applications. Prepared according to the formulas of Wickerham.

Manufacturer: bd™  B291930

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Catalog No. DF0919-08

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Description & Specifications


Media Format Nitrogen Source:  Ammonium sulfate 5g/L
Vitamins: Biotin 2μg/L, Calcium pantothenate 400μg/L, Folic acid 2μg/L, Inositol 2000μg/L, Niacin 400μg/L, p-Aminobenzoic Acid 200μg/L, Pyridoxine hydrochloride 400μg/L, Riboflavin 200μg/L, Thiamine hydrochloride 400μg/L
Compounds Supplying Trace Elements: Boric acid 500.0μg/L, Copper sulfate 40.0μg/L, Potassium iodide 100.0μg/L, Ferric chloride 200.0μg/L, Manganese sulfate 400.0μg/L, Sodium molybdate 200.0μg/L, Zinc sulfate 400.0μg/L
Salts: Monopotassium phosphate 1.0g/L, Magnesium sulfate 0.5g/L, Sodium chloride 0.1g/L, Calcium chloride 0.1g/L
Quantity 10kg
Format Dehydrated powder
Color White to off-white
Storage Requirements 2-8°C
Packaging Pail
Product Type Yeast Nitrogen without Amino Acids
Certifications/Compliance ISO, 9000
Media Type Yeast Nitrogen Base without Amino Acids
Shelf Life 1825 Days
For Use With (Application) For classifying Yeasts based on Nitrogen assimilation

Yeast Nitrogen Base without Amino Acids contains all essential vitamins and inorganic salts necessary for the cultivation of yeasts except histidine, methionine, tryptophan and a source of carbohydrate.

  • Prepared according to Guenter’s modification of Wickerham’s Yeast Nitrogen Base formulation
  • Good cultural response with Kloeckera apiculata 9774 and Saccharomyces cerevisiae 9080
  • pH (0.67% solution, 25°C): 5.4 ± 0.2

Yeasts are unicellular, eukaryotic, budding cells that are generally round-to-oval or elongate in shape. They multiply principally by the production of blastoconidia (buds). Yeast colonies are moist and creamy or glabrous to membranous in texture. Yeasts are considered opportunistic pathogens.


1825 Days