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BD™ Difco™ Mueller Hinton Broth

Used in the cultivation of a wide variety of fastidious and nonfastidious microorganisms. BD™ Difco™ Mueller Hinton Broth is a general purpose medium that is not cation adjusted. It may be used in antimicrobial susceptibility testing of microorganisms by broth dilution methods.

Manufacturer: bd™  B275730

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Catalog No. DF0757-17-6

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Description & Specifications


Format Dehydrated, powder
Color Light beige (with a few dark specks)
Quantity 500g
Packaging Bottle
Certifications/Compliance CE, CLSI
For Use With (Application) Cultivation of wide variety of fastidious and non-fastidious microorganisms
Media Format Beef extract 2g/L, Acid digest of Casein 17.5g/L, Starch 1.5g/L
Storage Requirements Refrigerate
Product Type Mueller Hinton broth
Media Type Mueller Hinton Broth
Shelf Life 1460 Days

Mueller Hinton Broth, not cation-adjusted, is a dehydrated culture medium that is not supplemented with calcium or magnesium ions. It is formulated to have a low thymine and thymidine content. It may be used for broth dilution antimicrobial susceptibility testing as long as the calcium and magnesium ion concentrations are adjusted according to CLSI standard M7.

  • Acid hydrolysate (digest) of casein and beef extract supply amino acids and other nitrogenous substances, minerals, vitamins, carbon and other nutrients to support the growth of microorganisms
  • Starch acts as a protective colloid against toxic substances in the medium; hydrolysis of the starch during autoclaving provides a small amount of dextrose, which is a source of energy
  • Displays a cultural response to: Enterococcus faecalis 29212, Escherichia coli 25922, Pseudomonas aeruginosa 27853 and Staphylococcus aureus 29213
  • pH (2.1% solution, 25°C): 7.3 ± 0.1
  • Calcium: 2.9–5.9mg/L; Magnesium: 3.2–5.2mg/L


1460 Days