The Difco™ LB Broth, Lennox media provide all of the nutritional requirements of organisms that have been derived from E. coli K12, which are deficient in B vitamin production. E. coli grows more rapidly in Lennox LB Broth than in conventional media because these media provide the cells with amino acids, nucleotide precursors, vitamins and other metabolites that the microorganism would otherwise have to synthesize.
LB Broth, Lennox contains ten times the sodium chloride level of Luria Broth Base, Miller and one half of that found in LB Broth, Miller. This allows the researcher to select the optimal salt concentration for a specific strain. If desired, the medium may be aseptically supplemented with glucose to prepare the complete medium described by Lennox.
- Formulation: Tryptone 10.0g, Yeast Extract 5.0g, Sodium Chloride 5.0 g
- Good cultural response to: Escherichia coli 33694 (HB101), Escherichia coli 39403 (JM103), Escherichia coli 53868 (DH5)
- To prepare, mix 20g in 1L distilled water and boil for 1 minute
- pH (3.5% solution, 25°C): 7.0 ± 0.2
|LB Broth, Lennox|
|Tryptone 10g/L, Yeast extract 5g/L, Sodium Chloride 5g/L|
|For maintaining and cultivating recombinant strains of Escherichia coli|