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BD Difco™ Chromogenic Dehydrated Culture Media: MI Agar

Culture media tests drinking water for the presence of E.coli and total coliforms with membrane filtration method

Manufacturer: bd  B214882

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Catalog No. B14882


Description & Specifications


Certifications/Compliance US, EPA
Color Beige
For Use With (Application) To detect and enumerate Escherichia coli and total Coliforms in drinking Water by the membrane filtration technique
Format Solid
Manufacturing Origin United States
Media Format No. 3 Proteose Peptone 5g/L, Yeast extract 3g/L, D-Lactose 1g/L, 4-Methylumbelliferyl-beta-D-Galactopyranoside 0.1g/L, Indoxyl-beta-D-Glucuronide 0.32g/L, Sodium Chloride 7.5g/L, Dipotassium Phosphate 3.3g/L, Monopotassium Phosphate 1g/L, Sodium Lauryl Sulfate 0.2g/L, Sodium Desoxycholate 0.1g/L, Agar 15g/L
Media Type MI Agar
Packaging Bottle
Quantity 100g
Shelf Life 730 Days
Storage Requirements 2-25°C

Detects degree of water quality deterioration in drinking distribution system enumerates, and monitors the progress in remediation. Provides precise and quantifiable results; also less time consuming and costly than other techniques.

  • Chromogenic-fluorogenic medium for detecting total coliforms and Escherichia coli in 24 hours or less
  • Under ambient light, E. coli exhibits bluish-gray appearance; under long-wave UV light, it displays blue-green fluorescence
  • Coliform are cream under ambient light, and display blue-white fluorescence under long-wave UV light
  • Conforms to EPA Method 1604 for monitoring drinking water under the Total Coliform Rule, and source water under the Surface Water Treatment


For testing ambient and potable water, treatment plant effluents, and beverage and pharmaceutical manufacturing