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Thermo Scientific™ B-PER™ Bacterial Protein Extraction Reagents

Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.

$269.85 - $751.80

Products
Catalog Number Mfr. No. Description Quantity Price Quantity    

PI78243

 
thermo scientific™
78243
B-PER Bacterial Protein Extraction Reagent 165mL Each for $269.85

PI78248

 
thermo scientific™
78248
B-PER Bacterial Protein Extraction Reagent 500mL Each for $483.00

PI78260

 
thermo scientific™
78260
B-PER II Bacterial Protein Extraction Reagent (2X) 250mL Each for $499.80

PI78266

 
thermo scientific™
78266
B-PER Bacterial Protein Extraction Reagent (in Phosphate Buffer) 500mL Each for $499.80

PI90078

 
thermo scientific™
90078
B-PER Bacterial Protein Extraction Kit 250mL kit Each for $422.10

PI90079

 
thermo scientific™
90079
B-PER Bacterial Protein Extraction Kit 500mL kit Each for $751.80

PI90080

 
thermo scientific™
90080
B-PER Direct Bacterial Protein Extraction Kit 50mL kit Each for $278.25

PI90084

 
thermo scientific™
90084
B-PER Bacterial Protein Extraction Reagent 250mL Each for $291.90
Description & Specifications

Perform efficient, gentle lysis and extraction of E.coli and other bacterial cells with Thermo Scientific™ B-PER Bacterial Protein Extraction Reagents.

These easy-to-use cell lysis reagents are nonionic detergent solutions that effectively disrupt cells and solubilize native or recombinant proteins without denaturation. Certain formulations include DNase I and lysozyme enzyme supplements to improve yield of large molecular weight proteins that are difficult to purify. B-PER Reagents are compatible with the addition of standard protease inhibitors and with downstream protein purification methods, such as GST purification and His-tagged immobilized metal affinity chromatography (IMAC).

Highlights:

  • Ready to use – B-PER Reagents provide one-step E.coli cell lysis by a mild, nonionic detergent (proprietary) in your choice of Tris or phosphate buffer formulations

  • Fast and simple – just add B-PER Reagent to a bacterial pellet, shake for 10 minutes and recover soluble proteins after pelleting the cell debris

  • Excellent yields – recover both soluble and insoluble recombinant protein from bacterial lysates and purify inclusion bodies to near-homogeneous levels

  • Flexible – B-PER Reagents are suitable for any scale of protein extraction and are available in phosphate and 1X and 2X Tris formulations, with and without enzymes

  • Compatible – completely compatible with addition of protease inhibitor cocktails, and resulting protein extract can be used in protein assays, typical affinity purification methods (e.g., GST, 6xHis) and other applications

Convenient, ready-to-use formats:

  • B-PER and B-PER II (2X) Reagents are free of enzymatic components
  • B-PER Reagent (in phosphate buffer) is an amine-free formulation for direct compatibility with labeling and crosslinking
  • B-PER with Enzymes and B-PER Direct with Enzymes include lysozyme and DNase I for improved lysis and recovery

Includes:

Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.