Arginase 1 Mouse anti-Human, Alexa Fluor® 488, Clone: sl6arg, eBioscience™
Mouse Monoclonal Antibody
Manufacturer: Life Technologies LS53977980
Description: The monoclonal antibody sl6arg recognizes human Arginase-1, a 35 kDa enzyme that that helps eliminate nitrogen by converting L-arginine to urea and L-ornithine which further converts to polyamine. These polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. Expression is found in the liver, neutrophils, as well as myeloid derived suppressor cells (MDSC) and neural stem cells. Expression in macrophages has not been determined in humans, while in mice expression in M2 macrophages is well established. Arginase-1 may be expressed in the myeloid population in breast cancer tumors and is typically found in the majority of hepatocellular carcinomas. Applications Reported: This sl6arg antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry. Applications Tested: This sl6arg antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.Arginase-1 (ARG1) catalyzes the hydrolysis of L-arginine into ornithine and urea, a critical step for the urea cycle. Defects in ARG1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia. There are several isoforms of mammalian arginase. The type I isoform, is a cytosolic enzyme and expressed predomitly in the liver as a component of the urea cycle. Hepatocellular carcinoma usually shows higher protein expression of ARG1 than normal liver cells.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|AI type I arginase; arginase 1, liver; arginase I; arginase, liver; arginase-1; liver-type arginase; type I arginase|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)|
|Alexa Fluor® 488|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.