Arginase 1, eFluor 660, clone: sl6arg, eBioscience™
Mouse Monoclonal Antibody
Manufacturer: Life Technologies LS50977982
Arginase-1 (ARG1) catalyzes the hydrolysis of L-arginine into ornithine and urea, a critical step for the urea cycle. Defects in ARG1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia. There are several isoforms of mammalian arginase. The type I isoform, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Hepatocellular carcinoma usually shows higher protein expression of ARG1 than normal liver cells.Description: The monoclonal antibody sl6arg recognizes human Arginase-1, a 35kDa enzyme that that helps eliminate nitrogen by converting L-arginine to urea and L-ornithine which further converts to polyamine. These polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. Expression is found in the liver, neutrophils, as well as myeloid derived suppressor cells (MDSC) and neural stem cells. Expression in macrophages has not been determined in humans, while in mice expression in M2 macrophages is well established. Arginase-1 may be expressed in the myeloid population in breast cancer tumors and is typically found in the majority of hepatocellular carcinomas.Applications Reported: This sl6arg antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry.Applications Tested: This sl6arg antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retreival and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|AI type I arginase, arginase 1, liver, arginase I, arginase, liver, arginase-1, liver-type arginase, type I arginase|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Microscopy|
|IgG1, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.