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Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor DFS Item

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA

$1,044.00 - $4,455.00

Specifications

Concentration 50 U/μL
Final Product Type First-Strand cDNA
Format First-Strand cDNA Synthesis Kit
GC-Rich PCR Performance High
PCR Method RT-PCR, RT-qPCR
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Products 2
Catalog Number Mfr. No. No. of Reactions Price Quantity  
Catalog Number Mfr. No. No. of Reactions Price Quantity  
4374967 DFS Item
View Documents Promotion Details
Applied Biosystems™
4374967
1,000 Reactions
Each for $4,455.00
 
4374966 DFS Item
View Documents Promotion Details
Applied Biosystems™
4374966
200 Reactions
Each for $1,044.00
 
Description

Description

The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in a single 20μL reaction. This kit also includes two tubes of RNase inhibitor (100μL each). Reactions can be scaled up to 100μL to generate 10μg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

  • Linear target amplification for real-time PCR
  • Higher yields and precision than other cDNA synthesis kits—at a fraction of the cost
  • Ten-fold greater dynamic range than other kits
  • Optimal reaction temperature: 37°C
  • Final product size: 7kb or less

Extensively tested with a variety of templates Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.

PCR & Real-Time PCR, Reverse Transcription

Specifications

Specifications

50 U/μL
First-Strand cDNA Synthesis Kit
RT-PCR, RT-qPCR
120 min.
RNA
100 μL, 20 μL
First-Strand cDNA
High
Separate components
Reverse Transcription
Reverse Transcription
SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.