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Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor DFS Item

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA

$628.00 - $2,850.00

Specifications

Sample Type RNA
Storage Requirements Store between -15deg. to -25deg.C
View More Specs

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.



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Catalog Number Mfr. No. Includes Quantity Price Quantity  
Catalog Number Mfr. No. Includes Quantity Price Quantity  
4374967 Life Technologies
LS4374967
10X RT Buffer (2 × 1mL); 10X RT Random Primers (2 × 1mL); 25X dNTP Mix (100mM, 1mL); MultiScribe Reverse Transcriptase (1mL at 50U/μL); RNase Inhibitor (10 × 0.1mL at 20U/μL) 1000 reactions Each for $2,850.00
4374966 Life Technologies
LS4374966
10X RT Buffer (1mL); 10X RT Random Primers (1mL); 25X dNTP Mix (100mM, 0.2mL); MultiScribe Reverse Transcriptase (0.2mL at 50U/μL); RNase Inhibitor (2 × 0.1mL at 20U/μL) 200 reactions Each for $628.00
Specifications

Description

The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in a single 20μL reaction. This kit also includes two tubes of RNase inhibitor (100μL each). Reactions can be scaled up to 100μL to generate 10μg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

  • Linear target amplification for real-time PCR
  • Higher yields and precision than other cDNA synthesis kits—at a fraction of the cost
  • Ten-fold greater dynamic range than other kits
  • Optimal reaction temperature: 37°C
  • Final product size: 7kb or less

Extensively tested with a variety of templates Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.

PCR & Real-Time PCR, Reverse Transcription

Description & Specifications

Specifications

RNA
Store between -15deg. to -25deg.C