Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor
Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA
Supplier: Applied Biosystems™ 4374967
The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in a single 20μL reaction. This kit also includes two tubes of RNase inhibitor (100μL each). Reactions can be scaled up to 100μL to generate 10μg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.
- Linear target amplification for real-time PCR
- Higher yields and precision than other cDNA synthesis kits—at a fraction of the cost
- Ten-fold greater dynamic range than other kits
- Optimal reaction temperature: 37°C
- Final product size: 7kb or less
Extensively tested with a variety of templates Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.
PCR & Real-Time PCR, Reverse Transcription
|Real Time PCR (qPCR)|
|Up to 7 kb|
|100 μL, 20 μL|
For Research Use Only. Not for use in diagnostic procedures.