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Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2 DFS Item

Chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation

$249.90 - $3,391.50

Specifications

For Use With (Application) Hot Start PCR, PCR, PCR and Real-Time PCR
Overhang 3'-A
Size (Final Product) 5kb or less
View More Specs

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Includes Quantity Price Quantity    

4311806

DFS Item
 
life technologies
4311806
1 tube (50μL at 5U/μL) of AmpliTaq Gold DNA Polymerase (250 units total); 1 tube (1.5mL) of GeneAmp™ 10X Gold Buffer (150mM Tris-HCl, pH 8.0, 500mM KCl); 1 tube (1.5mL) of 25mM magnesium chloride. 250 units Each for $249.90

4311814

DFS Item
 
life technologies
4311814
6 tubes (50μL at 5U/μL) of AmpliTaq Gold DNA Polymerase 6 tubes (1.5mL) of GeneAmp™ 10X Gold Buffer (150mM Tris-HCl, pH 8.0, 500mM KCl); 6 tubes (1.5mL) of 25mM magnesium chloride. 1500 units Each for $1,299.90

4311816

DFS Item
 
life technologies
4311816
1 tube (200μL at 5U/μL) of AmpliTaq Gold DNA Polymerase 4 tubes (1.5mL) of GeneAmp™ 10X Gold Buffer (150mM Tris-HCl, pH 8.0, 500mM KCl) 4 tubes (1.5mL) of 25mM magnesium chloride. 1000 units Each for $928.20

4311818

DFS Item
 
life technologies
4311818
5 tubes (200μL at 5U/μL) of AmpliTaq Gold DNA Polymerase (250 units total per tube); 20 tubes (1.5mL) of GeneAmp™ 10X Gold Buffer (150mM Tris-HCl, pH 8.0, 500mM KCl); 20 tubes (1.5mL) of 25mM magnesium chloride 5000 units Each for $3,391.50

4311820

DFS Item
 
life technologies
4311820
12 tubes (50μL at 5U/μL) of AmpliTaq Gold DNA Polymerase (250 units total per tube); 12 tubes (1.5mL) of GeneAmp™ 10X Gold Buffer (150mM Tris-HCl, pH 8.0, 500mM KCl); 12 tubes (1.5mL) of 25mM magnesium chloride. 3000 units Each for $2,357.25

4317742

DFS Item
 
life technologies
4317742
5 boxes total, each of which contains the following: 5 tubes (200μL at 5U/μL) of AmpliTaq Gold DNA Polymerase (250 units total per tube); 20 tubes (1.5mL) of GeneAmp™ 10X Gold Buffer (150mM Tris-HCl, pH 8.0, 500mM KCl); 20 tubes (1.5mL) of 25mM magnesium chloride 25,000 units CALL N/A N/A
 Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.
Description & Specifications

Specifications

For Use With (Application) Hot Start PCR, PCR, PCR and Real-Time PCR
Overhang 3'-A
Size (Final Product) 5kb or less

Hot-start activation
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Higher specificity, higher yield
AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination. GeneAmp 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold DNA Polymerase, resulting in a highly specific, robust PCR amplification. The ionic strength and the pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase.

AmpliTaq Gold 360 DNA Polymerase for superior performance
Also available is AmpliTaq Gold 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold DNA Polymerase, AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Supplied 360 GC Enhancer works through challenging GC-rich templates.

  • Time-released thermal activation improves sensitivity in low copy number amplifications
  • Successful multiplex PCR saves time and reagents
  • Includes GeneAmp 10X PCR Gold Buffer with MgCl2
  • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield

Hot Start PCR, PCR, PCR and Real-Time PCR

Shipping condition: Dry Ice