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Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2 DFS Item

Chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation

$238.35 - $3,570.00

Specifications

For Use With (Application) Hot Start PCR, Multiplex PCR, PCR, PCR and Real-Time PCR, Routine PCR
Overhang 3'-A
Size (Final Product) 5kb or less
View More Specs

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Includes Quantity Price Quantity    

N8080243

DFS Item
 
applied biosystems™
N8080243
6 tubes x 50μL each of AmpliTaq Gold DNA Polymerase (at 5U/μL, 250 units total per tube), 6 x 1.5mL of Buffer II (100mM Tris-HCl, pH 8.3, 500mM KCl), 6 x 25mM magnesium chloride. 1500 units Each for $1,276.80

N8080241

DFS Item
 
applied biosystems™
N8080241
50μL of AmpliTaq Gold DNA Polymerase (at 5U/μL, 250 units total), 1.5mL of Buffer II (100mM Tris-HCl, pH 8.3, 500mM KCl), and 1.5mL magnesium chloride Solution. 250 units Each for $238.35

N8080249

DFS Item
 
applied biosystems™
N8080249
5 tubes x 200μL each of AmpliTaq Gold DNA Polymerase (at 5U/μL), 1000 units total per tube. The enzyme also ships with 20 tubes x 1.5mL of Buffer II (100mM Tris-HCl, pH 8.3, 500mM KCl) and 20 tubes x 25mM magnesium chloride. 5000 units Each for $3,570.00

N8080247

DFS Item
 
applied biosystems™
N8080247
1 tube x 200μL each of AmpliTaq Gold DNA Polymerase (at 5U/μL, 250 units total), 4 x 1.5mL of Buffer II (100mM Tris-HCl, pH 8.3, 500mM KCl), 4 x 25mM magnesium chloride. 1000 units Each for $959.70

N8080259

DFS Item
 
applied biosystems™
N8080259
Contains 25 x 1000 units of AmpliTaq Gold DNA Polymerase (25,000 units total), Buffer II, and MgCl2 Solution. 25 x 1000 units CALL N/A N/A
 Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

N8080245

DFS Item
 
applied biosystems™
N8080245
12 tubes x 50μL each of AmpliTaq Gold DNA Polymerase (at 5U/μL, 250 units total per tube), 12 x 1.5mL of Buffer II (100mM Tris-HCl, pH 8.3, 500mM KCl), 12 x 25mM magnesium chloride. 3000 units Each for $2,310.00
Description & Specifications

Specifications

For Use With (Application) Hot Start PCR, Multiplex PCR, PCR, PCR and Real-Time PCR, Routine PCR
Overhang 3'-A
Size (Final Product) 5kb or less

Hot-start activation
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Higher specificity, higher yield
AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.

AmpliTaq Gold 360 DNA Polymerase for superior performance
Also available is AmpliTaq Gold 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold DNA Polymerase, AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Supplied 360 GC Enhancer works through challenging GC-rich templates.

  • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
  • Time-released thermal activation improves sensitivity in low copy number amplifications
  • Successful multiplex PCR saves time and reagents
  • Includes GeneAmp 10X PCR Buffer II with MgCl2

Hot Start PCR, Multiplex PCR, PCR, PCR and Real-Time PCR, Routine PCR

Shipping condition: Dry Ice