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anti-UNG, Polyclonal, Novus Biologicals™


Manufacturer: novus biologicals canada  NB600-1031SS

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 Disclaimers

For Research Use Only

Catalog No. NB6001031SS

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Description & Specifications

Specifications

Antigen UNG
Applications Immunocytochemistry
Applications Immunofluorescence
Applications Immunohistochemistry (Paraffin)
Applications Western Blotting
Concentration 0.5mg/mL
Conjugate Unlabeled
Cross Reactivity Human
Cross Reactivity Mouse
Cross Reactivity Rabbit
Cross Reactivity Rat
Format Purified
Formulation PBS containing 0.05% BSA
Gene Accession No. NP_550433
Host Species Rabbit
Immunogen This antibody was generated by immunizing rabbit with a synthetic peptide sequence CRHFSKTNELLQKSGKKP corresponding to amino acids 281-298 of human UNG1 (NP 003353.1) and amino acids 290-307 of human UNG2 (NP 550433.1). The peptide sequence used for immunogen is highly conserved between mammalian and invertabrates.
Quantity 0.025mg
Regulatory Status RUO
Primary or Secondary Primary
Monoclonal or Polyclonal Polyclonal
Gene ID (Entrez) 7374
Test Specificity The synthetic peptide sequence used for immunogen is 100% conserved between UNG1 and UNG2. Thus the antibody recognizes both UNG1 and UNG2. As UNG1 and UNG2 are not resolved as two species in gel electrophoresis (Huag et al., 1998), they are indistinguishable by western blot. The observed molecular weight of UNG (UNG1 and UNG2) on western blots may vary: 26 kDa, 27.5 kDa, 31 kDa, and 38 kDa forms have been described (Bharati et al., 1998; Slupphaug et al., 1993). Please see Kruman et al. (Fig. 1D, 2004) for an example of western blot in rat. This specificity of this antibody has been validated by antisense UNG oligonucleotide (Kruman et al. 2004). Cross reacts with Human, Mouse, Rat and Rabbit. Expected to cross-react with Chimpanzee, Dog, Zebrafish, Xenopus and Chicken.