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anti-TNF-alpha, Polyclonal, Novus Biologicals™

Manufacturer:  Novus Biologicals Canada NB600587SS

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Catalog No. NB600587SS


 

Disclaimers:

For Research Use Only


Specifications Description & Specifications

Specifications

TNF-alpha
Unlabeled
Antisera
Recombinant full length protein produced in E. coli (Human).
RUO
Polyclonal
The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNG beta (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Whole antisera; total protein concentration: 85mg/mL by Refractometry.
Rabbit
0.04mL
Primary
7124
Human,Murine,Primate,Rat