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anti-TNF-alpha, Polyclonal, Novus Biologicals™

Manufacturer: novus biologicals canada  NB600587

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For Research Use Only

Catalog No. NB600587

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Description & Specifications


Conjugate Unlabeled
Gene ID (Entrez) 7124
Monoclonal or Polyclonal Polyclonal
Formulation Whole antisera; total protein concentration: 85mg/mL by Refractometry.
Cross Reactivity Human
Cross Reactivity Mouse
Cross Reactivity Primate
Cross Reactivity Rat
Applications ELISA
Applications Immunohistochemistry
Applications Immunohistochemistry (Paraffin)
Applications Western Blotting
Regulatory Status RUO
Format Antiserum
Quantity 0.2mL
Antigen TNF-alpha
Immunogen Recombinant full length protein produced in E. coli (Human).
Primary or Secondary Primary
Test Specificity The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNG beta (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Host Species Rabbit