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anti-TEM8/ANTXR1, Clone: 200C1339(SB20), Novus Biologicals™

$139.00 - $321.00

Specifications

Clone 200C1339(SB20)
Host Species Mouse
Isotype IgG1
Immunogen This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 481-497 (CINFTRVKNNQPAKYPL) of human TEM8.
Antigen TEM8/ANTXR1
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 Disclaimers

For Research Use Only

Products
Catalog Number Mfr. No. Conjugate Quantity Price Quantity    

NB10056585

 
novus biologicals canada
NB100-56585
Unlabeled 0.1mg Each for $321.00

NB10056585T

 
novus biologicals canada
NB100-56585SS
Unlabeled 0.025mg Each for $139.00
Description & Specifications

Specifications

Clone 200C1339(SB20)
Host Species Mouse
Isotype IgG1
Immunogen This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 481-497 (CINFTRVKNNQPAKYPL) of human TEM8.
Antigen TEM8/ANTXR1
Monoclonal or Polyclonal Monoclonal
Format Purified
Primary or Secondary Primary
Regulatory Status RUO
Applications Immunocytochemistry
Applications Immunofluorescence
Applications Immunohistochemistry
Applications Immunohistochemistry (Paraffin)
Applications Western Blotting
Test Specificity TEM8 is observed at ∽80 kDa in western blots of HUVEC cells (Hotchkiss et al, 2005) and as an 80 kDa and 85 kDa double in human colon cell lysates (Nanada et al, 2004). The size difference is thought to be due to glycosylation as a 70 kDa band was seen when cell extracts in Nanda et al (2004) were treated with a glycosidase cocktail. Nanda et al (2004) note a passage dependent loss of TEM8 in HUVEC suggesting that that TEM8 may be a specific marker of the endothelial cell (EC) phenotype which is lost as primary EC's age in culture and de-differentiate. Reacts with Human. Based upon 94% immunogen sequence homology, this antibody is is predicted to react with Mouse and Rat also.
Gene Accession No. Q9H6X2
Gene ID (Entrez) 84168
Formulation PBS containing 0.05% BSA
Concentration 0.5mg/mL