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anti-TEM8/ANTXR1, Clone: 200C1339(SB20), Novus Biologicals™


Manufacturer: novus biologicals canada  NB100-56585SS

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For Research Use Only

Catalog No. NB10056585T

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Description & Specifications

Specifications

Antigen TEM8/ANTXR1
Applications Immunocytochemistry
Applications Immunofluorescence
Applications Immunohistochemistry
Applications Immunohistochemistry (Paraffin)
Applications Western Blotting
Clone 200C1339(SB20)
Concentration 0.5mg/mL
Conjugate Unlabeled
Cross Reactivity Human
Format Purified
Formulation PBS containing 0.05% BSA
Gene Accession No. Q9H6X2
Host Species Mouse
Immunogen This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 481-497 (CINFTRVKNNQPAKYPL) of human TEM8.
Isotype IgG1
Quantity 0.025mg
Regulatory Status RUO
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Gene ID (Entrez) 84168
Test Specificity TEM8 is observed at ∽80 kDa in western blots of HUVEC cells (Hotchkiss et al, 2005) and as an 80 kDa and 85 kDa double in human colon cell lysates (Nanada et al, 2004). The size difference is thought to be due to glycosylation as a 70 kDa band was seen when cell extracts in Nanda et al (2004) were treated with a glycosidase cocktail. Nanda et al (2004) note a passage dependent loss of TEM8 in HUVEC suggesting that that TEM8 may be a specific marker of the endothelial cell (EC) phenotype which is lost as primary EC's age in culture and de-differentiate. Reacts with Human. Based upon 94% immunogen sequence homology, this antibody is is predicted to react with Mouse and Rat also.