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anti-TEM8/ANTXR1, Clone: 200C1339(SB20), Novus Biologicals™


Manufacturer: novus biologicals canada  NB10056585

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For Research Use Only

Catalog No. NB10056585

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Description & Specifications

Specifications

Cross Reactivity Human
Clone 200C1339(SB20)
Applications Immunocytochemistry
Applications Immunofluorescence
Applications Immunohistochemistry
Applications Immunohistochemistry (Paraffin)
Applications Western Blotting
Isotype IgG1
Gene ID (Entrez) 84168
Gene Accession No. Q9H6X2
Monoclonal or Polyclonal Monoclonal
Formulation PBS containing 0.05% BSA
Conjugate Unlabeled
Primary or Secondary Primary
Quantity 0.1mg
Antigen TEM8/ANTXR1
Test Specificity TEM8 is observed at ∽80 kDa in western blots of HUVEC cells (Hotchkiss et al, 2005) and as an 80 kDa and 85 kDa double in human colon cell lysates (Nanada et al, 2004). The size difference is thought to be due to glycosylation as a 70 kDa band was seen when cell extracts in Nanda et al (2004) were treated with a glycosidase cocktail. Nanda et al (2004) note a passage dependent loss of TEM8 in HUVEC suggesting that that TEM8 may be a specific marker of the endothelial cell (EC) phenotype which is lost as primary EC's age in culture and de-differentiate. Reacts with Human. Based upon 94% immunogen sequence homology, this antibody is is predicted to react with Mouse and Rat also.
Regulatory Status RUO
Immunogen This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 481-497 (CINFTRVKNNQPAKYPL) of human TEM8.
Format Purified
Host Species Mouse
Concentration 0.5mg/mL