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CD278 (ICOS) Armenian Hamster anti-Mouse, Rat, Rhesus Monkey, FITC, Clone: C398.4A, eBioscience™

Armenian Hamster Monoclonal Antibody

Manufacturer:  Life Technologies LS11994982

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Catalog No. 501128700



Description

Description

Description: The C398.4A monoclonal antibody reacts with ICOS (Inducible Costimulatory molecule), also known as H4/CRP-1/AILIM, and cross-reacts with mouse and rat ICOS. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. A dimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1, is thought to play a protective role in inflammatory autoimmune diseases and be involved in the development of Th2 cells. C398.4A is reported to display strong costimulation of proliferation of T cell clones, intermediate costimulation on activated T cells, and weak costimulation on fresh resting T cells. It is a good costimulator of IL-10 production especially from pre-activated cells that express high level of H4/ICOS. The epitope recognized by C398.4A is different from that bound by the ICOSL (i.e., C398.4A does not block binding of ICOSL). Applications Reported: The C398.4A antibody has been reported for use in flow cytometric analysis. Applications Tested: The C398.4A antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T-cells. It is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells.
Specifications

Specifications

CD278 (ICOS)
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q9R1T7, Q9WVS0
AILIM, CD278, CVID1, activation-inducible lymphocyte immunomediatory molecule, inducible T-cell costimulator, inducible costimulator
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse, Rat, Rhesus Monkey
Flow Cytometry
C398.4A
FITC
Icos
Liquid
ICOS
IgG
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
54167, 64545, 705788
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