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anti-PMEL17/SILV, Clone: HMB45, Novus Biologicals™

Manufacturer: novus biologicals canada  NBP229418

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For Research Use Only

Catalog No. NBP229418

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Description & Specifications


Conjugate Unlabeled
Format Concentrated
Applications Flow Cytometry
Applications Immunocytochemistry
Applications Immunofluorescence
Applications Immunohistochemistry (Frozen)
Applications Immunohistochemistry (Paraffin)
Applications Immunoprecipitation
Applications Western Blotting
Isotype κ
Regulatory Status RUO
Quantity 0.5mL
Clone HMB45
Host Species Mouse
Cross Reactivity Human
Antigen PMEL17/SILV
Gene ID (Entrez) 6490
Primary or Secondary Primary
Gene Accession No. P40967
Formulation Culture Medium
Monoclonal or Polyclonal Monoclonal
Test Specificity By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. Anti-HMB-45 stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa). Canine (Negative), Feline (Negative).
Immunogen An extract of pigmented melanoma metastases from lymph nodes was used as the immunogen for the melanoma HMB45 antibody.