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anti-p53, Clone: DO7, Novus Biologicals™


Manufacturer: novus biologicals canada  NBP229419

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 Disclaimers

For Research Use Only

Catalog No. NBP229419

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Description & Specifications

Specifications

Antigen p53
Applications Flow Cytometry
Applications Immunocytochemistry
Applications Immunofluorescence
Applications Immunohistochemistry (Frozen)
Applications Immunohistochemistry (Paraffin)
Applications Immunoprecipitation
Applications Western Blotting
Clone DO7
Conjugate Unlabeled
Cross Reactivity Bovine
Cross Reactivity Human
Cross Reactivity Primate
Format Concentrated
Formulation Culture Medium
Gene Accession No. P04637
Host Species Mouse
Immunogen A recombinant protein fragment specific to p53 was used as immunogen for the DO7 antibody.
Isotype κ
Quantity 0.5mL
Regulatory Status RUO
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Gene ID (Entrez) 7157
Test Specificity Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. Its epitope maps within the N-terminus (aa 37-45) of p53. Monoclonal antibody PAb1801 does not block the binding of DO-7 MAb to p53 in an ELISA test. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.